Yes. VO2 Vegan Cafe (O2 yoga) in Boston has an all-gender restroom.
1001 Massachusetts Ave., Cambridge, Boston , MassachusettsVerifiedby community-sourced import · Mar 1, 2017How checks work
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Restroom
All-gender: Yes · Wheelchair access: ADA accessible
On the first floor between cafe and massage parlour Large single stall. Contains a chalk board. I assume it is for paying customers at Cafe, massage parlour or yoga classes. However the lovely people who work there will likely let you use it if you're desperate.
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32363updated upstream Mar 1, 2017