Yes. Prezzemolina in Rome has an all-gender restroom.
Via del Colosseo, 1, 00184, Rome, LazioVerifiedby community-sourced import · Nov 1, 2021How checks work
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Restroom
All-gender: Yes
First floor on the left behind the counter May have to be a paying customer. The door doesn't lock but the staff will tell others it's occupied for you. There's no toilet seat.
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60339updated upstream Nov 1, 2021