Yes. New England Aquarium in Boston has an all-gender restroom.
Central Wharf, Boston, MAVerifiedby community-sourced import · Feb 2, 2014How checks work
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Restroom
All-gender: Yes · Wheelchair access: ADA accessible
The bathrooms on the 2nd floor, next to the cafe, are single-person locking. They are marked as \\\"family\\\" bathrooms, but individuals without kids use them all the time. As far as getting to them - you can actually go to the cafe (and therefore the bathrooms) without paying. Unless you are in a wheelchair - it is only elevator accessible from inside the Aquarium exhibit halls.
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2822updated upstream Feb 2, 2014 · 3 up, 1 down on Refuge