Yes. Jimmy John's in Charlotte has an all-gender restroom.
16051 Lancaster Hwy, Charlotte, North CarolinaVerifiedby community-sourced import · Sep 4, 2016How checks work
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All-gender: Yes · Wheelchair access: ADA accessible
It's all the way in the back of the store, not visible to employees or many patrons (plus few people dine in here). You probably have to be a paying customer because you do have to walk past the counter to get to where the bathrooms are.
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25326updated upstream Sep 4, 2016