Yes. Iowa 80 Truckstop ("World's Largest Truckstop") in Walcott has an all-gender restroom.
755 W Iowa 80 Rd, Walcott, IowaVerifiedby community-sourced import · Apr 8, 2016How checks work
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Restroom
All-gender: Yes · Wheelchair access: ADA accessible
Restrooms are located near Caribou Coffee in the food court area. The gender neutral family restroom is in the same hallway as the standard restrooms. No restrictions on access or use.
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