Yes. I-75 NB Rest Area at MM 413 in Lake Butler has an all-gender restroom.
I-75 Northbound, Mile 413, Lake Butler, FLVerifiedby community-sourced import · Feb 2, 2014How checks work
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Restroom
All-gender: Yes · Wheelchair access: ADA accessible
In center of building, between the men's wing and the women's wing. Slight lip at the door, which is relatively light for being made of metal. The lock is another of those stubby little keys that turn a deadbolt, but requires only a 45-degree turn and little force.
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2255updated upstream Feb 2, 2014 · 0 up, 1 down on Refuge