Yes. I-75 NB Rest Area at MM 383 (Paynes Prairie) in Gainesville has an all-gender restroom.
I-75 Northbound, Mile 383, Gainesville, FLVerifiedby community-sourced import · Feb 2, 2014How checks work
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Restroom
All-gender: Yes · Wheelchair access: ADA accessible
On the women's side of the building, to the left and right of the entrance to the hallway leading to the women's communal restrooms. Available 24/7. Moderately accessible; the door is heavy and shuts itself, but the lock is small and stubby and requires more than a full 360-degree turn to close the deadbolt. There is a slight lip in the doorway plate which may require a bit more strength than a flat entrance, which does not combine well with the heavy, self-closing door.
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3033updated upstream Feb 2, 2014 · 0 up, 1 down on Refuge