Yes. Humane Society of Boulder Valley in Boulder has an all-gender restroom.
2323 55th Street, Boulder, ColoradoVerifiedby community-sourced import · Jun 8, 2017How checks work
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Restroom
All-gender: Yes · Wheelchair access: ADA accessible
Single room marked "unisex" on second floor (stairs/lift between the service desk and clinic area, turn right and right around the wall, past the staff lounge when you get upstairs).
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35589updated upstream Jun 8, 2017