Yes. Dollar Tree in Ancaster has an all-gender restroom.
1100 Wilson St W, Ancaster, Ancaster, OntarioVerifiedby community-sourced import · Jul 16, 2016How checks work
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Restroom
All-gender: Yes
Back of the store to the right, go through the door, then the first door on the right. Room is an unmarked single stall.
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24096updated upstream Jul 16, 2016